Show simple item record

dc.creatorKosovac, Andrea
dc.creatorJakovljević, Miljana
dc.creatorKrstić, Oliver
dc.creatorCvrković, Tatjana
dc.creatorMitrović, Milana
dc.creatorToševski, Ivo
dc.creatorJović, Jelena
dc.date.accessioned2019-04-04T16:07:39Z
dc.date.available2019-04-04T16:07:39Z
dc.date.issued2019
dc.identifier.issn0929-1873
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/565
dc.description.abstractBois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.en
dc.publisherSpringer, Dordrecht
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.relationSCOPES program of the Swiss National Science Foundation - IZ73Z0_152414
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Plant Pathology
dc.subjectGenetic differentiationen
dc.subjectHost-plant associationsen
dc.subjectInsect-vectoren
dc.subjectMitochondrial COI gene markeren
dc.subjectMultilocus genotypingen
dc.subjecttufen
dc.subjectstampen
dc.subjectvmp1en
dc.subjectStolbur phytoplasmaen
dc.subjectVector roleen
dc.titleRole of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbiaen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage195
dc.citation.issue1
dc.citation.other153(1): 183-195
dc.citation.rankM21~
dc.citation.spage183
dc.citation.volume153
dc.type.versionpublishedVersion
dc.identifier.doi10.1007/s10658-018-1553-1
dc.identifier.rcubconv_1029
dc.identifier.scopus2-s2.0-85050608074
dc.identifier.wos000461686500001


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record